Bellerophon is a hybrid split-read and paired-read method for detecting and classifying interchromosomal structural variants at base-pair level. These interchromosomal variants may include reciprocal and non-reciprocal translocations, and also interchromosomal insertions (i.e. the donation of a contiguous chromosomal segment to another). Like other types of variation, interchromosomal variants may disrupt gene function or result in the formation of oncogenic fusion genes.
Bellerophon was developed in a Unix environment and requires the installation of Perl. It also requires the separate download of the following programs: BLAT, SAMTools, and ReadBackwards.pm (a perl module for reading files backwards). The perl module is used to find potential q-arm/q-arm chromosomal fusions. The links to each program are provided on this page.
Changes from version 1.01
- Fixed a bug in bellerophon.pl that prevented records from being filtered out for having an insufficient amount of soft-clipped reads.
- Fixed a bug in bellerophon.pl that erroneously disabled user-specified mean and standard deviation option.
- Fixed a bug in classify.pl that placed some predicted records on the same line in the output.